Journal: Oncology Letters

Article Title: ZAP70 interaction with 13 mRNAs as a potential immunotherapeutic target for endometrial cancer

doi: 10.3892/ol.2023.13799

Figure Lengend Snippet: In vivo and in vitro validation of ZAP70 expression and its potential role in prognosis. (A) ZAP70 mRNA expression was detected in tissues by reverse transcription-quantitative PCR. (B) ZAP70 protein expression was detected in tissues by immunohistochemistry. Image intake was presented through the microscope (cat. no. 703548; Nikon Corporation). (C) Localization of ZAP70 in cells was detected using immunofluorescence (400×). Image intake was presented through the microscope (cat. no. 703548; Nikon Corporation). Differences in ZAP70 between (D) healthy and tumoral tissues, (E) FIGO clinical stages I/II and stages III/IV, and (F) living and deceased patients. IHC, immunohistochemistry; OS, overall survival; TPM, transcripts per kilobase million; ZAP70, ζ-chain-associated protein kinase 70. *P<0.05 and ***P<0.001.

Article Snippet: The 2-step plus Poly-HRP Anti Rabbit/Mouse IgG Detection System immunohistochemistry kit (cat. no. E-IR-R211; Elabscience Biotechnology, Inc.) was used to analyze tissue sections (4 μm).

Techniques: In Vivo, In Vitro, Biomarker Discovery, Expressing, Reverse Transcription, Real-time Polymerase Chain Reaction, Immunohistochemistry, Microscopy, Immunofluorescence